Caliban’s Feelings Towards Propero Essay

In this essay I will tell you about Caliban’s feelings towards Prospero, how Prospero treats Caliban and a chain of exploitation involving Caliban and Prospero. Caliban is the rightful owner of an island that Prospero, an ex-duke of Milan and a type of sorcerer, and his daughter Miranda, a girl who was only 3 years old when her father was overthrown by his brother Antonio and pities people who are harmed by her father’s magic, took over when they arrived on the island and managed to control Caliban with his magic. Caliban finds more people to work with, when Prospero ship wrecked their boat, called Trinculo and Stephano and plan to kill Prospero. After Prospero took over the island Caliban has tried to reclaim it but is then punished by Prospero’s magic. When Caliban talks to Trinculo and Stephano about trying to reclaim the island from him he tells how he was usurped ‘As I told thee before, I am subject to a tyrant, a sorcerer, that by his cunning hath cheated me of the island’. The words tyrant and sorcerer display Prospero as an evil and cruel master, when Caliban calls him a tyrant means that he thinks Prospero is a person who exercises power in a harsh, cruel manner. In the quote it says he was ‘cheated me of the island’ shows that he was ill-treated by Prospero and suggests that Caliban has been made inferior. In line 106 Caliban asks Stephano if he will destroy Prospero, the use of the word destroy tells the audience that Caliban hates Prospero so much he doesn’t only want to kill him he wants to hurt him so much that the broken beyond repair, and he wants them to ‘batter his skull†¦Ã¢â‚¬ ¦.  Cut his wezand (his windpipe/throat)†¦Ã¢â‚¬ ¦paunch him’, punching is when you cut an animal’s stomach area and all its intestines fall out and you then clean the intestines to get out the good meat. The way Prospero treats Caliban according to Caliban he is ill-treated by Prospero and tells Stephano the ways he is cruel to him, ‘I say by sorcery he got this isle, from me he got it†¦Ã¢â‚¬ ¦..  Revenge on him for I know thou dar’st’, when Caliban tells Stephano that he was usurped by cruel magic he asks ‘how now shall this be compassed? Canst thou bring me to the party’, Stephano is asking Caliban how will he get his revenge and can he come along to help Caliban. Stephano agrees with Caliban that he was ill-treated and happily agrees the help kill Prospero and in return he gets to rule the island and have Caliban and Trinculo as his viceroys. While Caliban is telling Stephano of all the horrid things he wants to do to Prospero he tells him about his daughter Miranda and Caliban says ‘Ay, lord, she will become thy bed, I warrant, and bring thee forth brave brood’ the exploitation is that Caliban is telling Stephano that he can force Miranda to sleep with him and give birth to many children at his pleasure, the irony in this is that Caliban exploiting Miranda, the person who has always been exploited. When Caliban asks Stephano if he will help kill his master he says ‘beat him enough, after a little time I’ll beat him too’ the exploitation in this quote is to Prospero because they are implying that they can easily overpower him and beat him, in line 106 Caliban asks Stephano ‘Wilt thou destroy him then’, the word destroy tells that he does not only think he can overpower him but have enough strength to destroy him, this exploitation is no ironic because Prospero used his strength to exploit Caliban. Whilst Caliban is listing the ways he wants to kill Prospero he tells Stephano and Trinculo ‘First possess his books: for without them he’s but a sot’ He is telling them this because he knows how weak he can be without his magic and his books and how strong he is with them. The fact that Caliban knows his weaknesses tells the audience that he has been planning to get revenge on his master, now he has formed an alliance with Stephano and Trinculo means that he can put his plan into action and finally get revenge on his old cruel master Prospero. He follows on from this and says ‘burn but his books; he has brave utensils- for so he calls them’ Caliban is saying that you can burn anything you want except his books because they hold great power and will be a type of trophy they gain for killing Prospero. In the story of The Tempest I think that Caliban and Prospero’s relationship is bad because at the beginning they argue about whose island it is. Later on in the story Caliban finds Stephano and Trinculo and makes an alliance with them, he then promises to show and tell them the best springs are in return for some wine, he also gets them to help him get his revenge on Prospero. Prospero doesn’t like Caliban because he abuses Miranda and always threatens him and tries not to help them live their lives. Overall I think Prospero and Caliban’s relationship is the worst in the whole story and doesn’t improve and only gets worst.

Nathaniel Hawthorne Essay

For the American writer Nathaniel Hawthorne the most explored theme of all his writings is the imperfect spirituality of man and the pervasiveness of sin throughout creation. Both of the stories under analysis here, Young Goodman Brown and The Minister’s Black Veil, feature a young reverend as the central character of the work and a Puritanical community in New England as the setting. Both of the tales are allegories centered on the ambiguity of human spirituality and on the ubiquity of sin in creation. Young Goodman Brown is an allegory about the deep mystery of sin, for which the author makes use of all available suggestive elements, from the setting- a deep and gloomy forest in New England, to the characters and their symbolic names- Goodman, an obvious hint at â€Å"good man†, and Faith, an equally transparent hint at religious faith, to other symbolic elements such as the staff resembling a wrinkled serpent, obviously the serpent in the Garden of Eden. Hawthorne approaches the idea of sin in his allegorical usual way, with conspicuous Biblical allusions, but also with deep psychological insight into the character’s soul and mind. He delves profoundly into human consciousness and the life of the mortal soul, highlighting the permanent conflicts between virtue and sin. In Young Goodman Brown we follow the main character on his intricate course through the dark and gloomy forest, reminding us of the Garden of Eden, and we are confronted with the grim temptations of sin in a vague, confusing setting, where the line dividing-line between dream and reality becomes very thin. It is Hawthorne’s own choice to leave the question as to whether the night’s confused events and the meeting of the community of witches was a dream or reality. Through this artifice, an essential problem is set forth- the result is that the reader has to wonder whether the sin and the fall into temptation belong to Goodman alone or whether the entire community is pervaded by sinfulness and immorality. The forest is thus a symbol for the human consciousness and for the spiritual life of man, and what Hawthorne undertakes to find out is whether everybody is actually lost on the paths of the spiritual, and which of the two –virtue or sin – is the true state of the spiritual life. Another important aspect is that in the midst of the gloominess of the forest meet for initiation into the lore of sinfulness, both the villagers who are commonly considered as virtuous and pious in everyday life, and are often set an example, and those members of the community who are normally viewed as sinners or criminals. Thus, the two main sides of spiritual life- virtue and sin are both mere appearances, whereas in the inner life of man they live together undisturbed. The Minister’s Black Veil is similar to Young Goodman Brown in many respects. Again, the centre of the story is the tormented consciousness of a young priest, who chooses to wear a symbolic black veil over his eyes, that prevents him from enjoying any kind of mortal happiness. In the end, the veil is seen as a symbol for the darkness and sinfulness that is hidden into the depths of human consciousness and that separates man from his fellows. As Goodman Brown typifies the average man, torn between virtue and sin, the minister in this story symbolizes the imperfect spirituality of man. The black veil that hides the face of the young priest is but the symbol of the outward representation of sin. According to Hawthorne, sin is inherent in the soul and is only veiled by the appearance of virtue. Sinfulness lies therefore deep within and separates all men from one another as the black veil separates the minister both from light and from communion with his fellows. Hawthorne therefore tackles mortal imperfection and the pervasive nature of sin in creation. Both stories emphasize the nature of human spirituality, at the very core of which sin and unlawfulness thrive.

Employment Law Essay Example | Topics and Well Written Essays - 1000 words

Employment Law - Essay Example 1). The employment issue, which exists, is whether it is right for resumes of unemployed people to be discarded by employers. An operations analyst in the information technology sector is an example of a person who is discriminated by the employment act. According to a recruiter, not working for six months is an obstacle towards finding employment. This ideology seems to have spread to almost all the American employers. Furthermore, the number of Americans who do not have jobs is at 14 million, and finding work is a problem. Some of America’s known Job websites such as Craigslist, CareerBuilder, and Monster.com are not employing the unemployed. This situation has a historical context like that, which took place during the Great Depression. During that time, many workers were left unemployed, and this is due to the crisis. It does not make sense when employers demand that potential recruits be currently employed. There are some people who are fresh out of college and have never worked, yet they have the skills and knowledge. They should be given a chance to prove their capability, and thus, this employment discrimination is problematic (Rampell, 2011. 1). According to a legal view, like race and age there is no protected status in matters related to employment. Experts further believe that there is no discrimination that occurs as a result of this practice. The employment laws, which exist, are not violated in any way. It has emerged that the most vulnerable groups affected by this practice are blacks and elderly people. The latter is according to information provided by Equal Employment Opportunity Commission (EEOC). The latter has not yet decided whether the case on the unemployed is illegal in any way. In fact, they have hearings on this matter, which are taking place at the moment. The unemployed are crossing their fingers, in order for a law to be passed concerning this matter (Livingston et al, 20005. 114). Employment discrimination has become a men ace, and a law has been passed by New Jersey concerning this matter. Other states such as Michigan and New York are considering having such a law. The economy is being affected, as there are no people who are employed to earn revenue for the economy. There will no longer be a scenario where unemployed workers are discouraged from applying due to their unemployment status. The plight of workers is being addressed by the National Employment Law Project (Decker, 2002. 42). It advocates for politicians to find ways to outlaw adverts that discriminate against the unemployed workers. Furthermore, it advocates for the unemployed to obtain benefits, as well as engage in the labor market studies. Almost all professions are involved in this discriminative practice, and including even top American companies. It seems that this matter cannot be solved easily as Congress is not that influential. Companies are the ones who have the upper hand in this matter, since they are the ones who hire indiv iduals. They choose the people they want, and congress cannot interfere. If the matter is taken to court, proving that discrimination occurred will be extremely hard. There is enough evidence to prove that workers who have stayed for long without being employed may not be beneficial to the work place. Some of the reasons why they are unemployed is because they lack competence, and are laid off as there is no work. In some

The State Should Take No Part in Employment Relations, Leaving It Essay

The State Should Take No Part in Employment Relations, Leaving It Entirely to Employers and Their Employees - Essay Example It is understandable that there are certain laws that have helped the workers in ensuring a stable income and has provided job security also. On the other hand, there are issues regarding the coalition’s perspective because it is believed that it is important to create legislation for supporting the success of businesses (Accel Team 2007). Therefore, the aspect from both the sides will be evaluated in context with the interference of the state in the employment relations. During the past two decades, the reform of employment relations has been considered as very significant to political and economic debate. In late 1980s, the process of enterprise bargaining was introduced by Hawke Labor government, with the support of union government, in order to decentralize the employments relations system. This paper will also analyze the modern workplace of Australia regarding employment relations. Arguments in favor of the state intervention It has been observed that the role of governm ent on the employment relations is very significant as it helps in setting up a legal framework that industrial relations operate. A proper legislation helps in identifying the requirements of both employees and employers, because the fact is that the employees and the employers both want to benefit from each other as they are reliant on each other. This states that it is necessary to recognize the equal bargaining power of the workers and the employers. The laws of employment relations should be appropriately implemented addressing any imbalance of power and both groups should be given equal degree of control. Proper legislation on the employment relations should allow a mixture of both collective and individual bargaining, as well as it should also support in facilitating employee participation in the regular decisions taken at the workplace. The state provides a structure and framework for employment relationship, which is formally controlled by the legislation to secure good emp loyment relations (Combet 2005). Australian modern workplace The main debate regarding the industrial relations in Australia is to organize and manage the labor practices. On the other hand, there is a difference in opinion of both the current government and the opposition in relation with the present industrial relations laws, and the main issues are concerned with the use of the third parties or unions, individualist and collectivist frames of reference and individual vs. collective bargaining. The proper employment relation laws are to protect minimum wage, outlaw discrimination, prevent the abuse of power by either party, and determine minimum standards of safety, health, hygiene, and minimum employment conditions (Bailyn and Fletcher 2002). In order to determine pay and other key conditions of the employment, government has used legislation to establish industrial tribunals tasked with the role in the past. The state has also played a key role in preventing and settling industr ial disputes. In the earlier times, the center of attention was on collective bargaining through unions and employers, in the region of minimum wages and conditions laid down by the tribunal. This model was created on the whole purpose and did not acquaint with the individual requirements of both employees and employers. But, presently, the legislation has altered to try and support better flexibility among workers and managers (Crosby 2002). If it is analyzed from both the

Biodegradability of polymers Term Paper Example | Topics and Well Written Essays - 750 words

Biodegradability of polymers - Term Paper Example The degradable products are biogas, carbon dioxide and methane. Natural polymers such as polysaccharides, proteins and nucleic acid are degraded by hydrolysis and oxidation. Biodegradation can occur aerobically and anaerobically depending on the availability of oxygen. Chemical structure, physical properties, molecular weight, porosity, elasticity and morphology are responsible for a material’s biodegradation. Compounds which can be biodegradable have the power to break down in environments where the material is decomposed by biological processes with the production of water, carbon dioxide and carbon containing soil (Leja and Lewandowicz 256). There are various tests which can be performed to assess the biodegradability of polymers. The tests performed can be simple or complex depending on their duration of action. Simple tests are rapid and complex ones are slow. The test methods that are practiced are different. Plate testing method is a simple method used as an initial test. The polymer that is to be tested is set on a mineral salt agar accompanied with some carbon for fungal growth to start. Then the sample is inoculated by fungi such as Aspergillusniger. The level of biodegradability is measured by loss of dry weight and surface colonization. Another method for assessment is the enzyme test. In this method specific enzymes are used to catalyze polymers such as starch-blended polymers. The polymer of choice is inserted into an enzyme solution and is incubated. The degree of biodegradability is measured by weight loss of polymer. Another method is when various microbial consortia or groups are identified for various po lymers. This test gives its assessment by showing changes in molecular weight and mechanical properties. Modified sturm test is a simple method in which activated sewage sludge is used. The desired

The Development of African Dance Essay Example | Topics and Well Written Essays - 500 words

The Development of African Dance - Essay Example There are important aspects of African dance and music that have evolved into the modern times and greatly influenced contemporary art forms worldwide. For example, rap music and hiphop dance, while they are very much part of contemporary art forms of the youth, are new versions of traditional African culture.African dances are full of symbolism. One persistent aspect of African dance is a common dance-pattern that is depicted by a wide, sweeping circle around a group of musicians situated in the center. Despite historical changes to dance, the circle formation has proved to be very durable, despite attempts to break up the circle formation. James (2000) says that the moving circle that defines the inner space for the dancers is a symbol of the celebration of the culture’s distinctiveness, and this symbol could be traced through Africa’s history of political subjugation and social change. The pattern could also be found in dances in slave plantations in the New World, a s well as the Sudanese and Ethiopian region of the upper Blue Nile.The circle formation could is evident in the Capoeira, an Afro-Brazilian art form which ritualizes movements from martial arts and games and combines these with dance. (Please see powerpoint presentation.) Capoeira was popularized as a Brazilian dance, but evidence supports that it is a folk dance form developed by African slaves from traditional African dances and rituals. While African dance has influenced dance in other cultures, some authors such as Loots (2006).

Miranda v. Arizona -what was the out come of the case and why i chose Term Paper

Miranda v. Arizona -what was the out come of the case and why i chose to report on it - Term Paper Example In short, this case succeeded in writing a new chapter in the criminal justice system in America which forced me to select this case and to write about it. A kidnapping and sexual assault occurred in Phoenix, Arizona, in March 1963. On March 13 Ernesto Miranda, 23, was arrested in his home, taken to the police station, identified by the victim, and taken into an interrogation room. Miranda was not told of his rights to counsel prior to questioning. Two hours later, investigators emerged from the room with a written confession signed by Miranda. It included a typed disclaimer, also signed by Miranda, stating that he had â€Å"full knowledge of my legal rights, understanding any statement I make maybe used against me,† and that he had knowingly waived those rights. Two weeks later at a preliminary hearing, Miranda again was denied counsel. At his trial he did have a lawyer, whose objections to the use of Miranda's signed confession as evidence were overruled. Miranda was convict ed of kidnapping and rape, and received a 20-year sentence (Miranda v. Arizona (1966)) The above case raised lot of constitutional issues. According to The Fifth Amendment to the United States Constitution, an accused or a witness has the right to protect himself from being forced to incriminate. In other words, the accused has the right to stay silent in police custody or to consult with a counsel before answering the questions of the investigating officer. In other words The Fifth Amendment to the United States Constitution gives a privilege against self-incrimination to the suspect or accused. Earlier, prior to this case, the investigating officers in United States were using all types of physical and psychological tortures to force the accused to accept the crime. They used circumstantial evidences to arrest the suspected people when a crime occurs. However, circumstantial evidences need not be hundred percent correct in all criminal cases. It is quite possible that a smart crim inal can commit crimes and mislead the investigating officers towards innocent people with the help of created evidences. If the police officer was not smart enough, he will take those circumstantial evidences or evidences collected from the spot seriously and arrest the innocent person. If that person was unaware of his rights in police custody, he may confess the crime in order to escape from the brutal interrogation methods of police. Thus, it is quite possible that an innocent may be punished by the court. According to the criminal justice system in America, not even a single innocent person should be punished even if thousands of criminals escaped from punishment. In other words, the criminal justice system urges the investigating officers to thinks twice or thrice before arresting person for a criminal activity. An accused has been given the right to consult with a lawyer before answering the questions of an investigating officer. It is quite possible that a smart investigatin g officer may ask misleading questions to the accused and force the accused to accept the crime. The investigating officer may have better knowledge about the criminal procedures than the accused and he can utilize that knowledge effectively to make an innocent person, a criminal. In order to avoid such

Management Theory Essay Example | Topics and Well Written Essays - 1250 words

Management Theory - Essay Example Many theories are formulated for different schools of thoughts with the help of fathers of Management. There are different management theories main are Scientific management theory, it clearly focus on the maximizing the efficiency by matching the job description with people; other is administrative management with clearly enlighten the creation of efficient systems in management and in organization. Then comes the behavioral management theories, these are developed both pre and post Second World War, this theory focus on the phenomenon of controlling the work force and leading them in a way to increase the performance. Lastly the management science theory that was born during Second World War and gained popularity in researchers, this theory focus on the measuring and controlling of organizations performance through quantitative and analytical techniques done by managers. To deal with formal organization and the concepts for enhancing the management efficiency Classical organization theories were born by Taylor formed scientific management concepts in 1947, Weber introduced the bureaucratic approach in 1947 and Fayol presented the administrative theory of the organization in 1949. These three management fathers have contributed a lot in the developing on classical theories. Adam smith was the founder of management theories, he observed two factories making pin wheel. In one factory a worker had to perform 18 tasks on his own to make a pin wheel and could make few thousands of pin wheel by the end of the day, where as in other factory 10 workers has expertise on one task and were able to manufacture 48,000 pin wheel per day. This gave birth to the idea of Job Specialization, it means that if workers are skilled in one tasks then and will do it repeatedly, they will have safe hands and will have faster rate of production with same quality. It will increase the efficiency and will lead to great organizational performance. Era of modern management was begun with t he theories of Fredric Taylor, when he noticed the national loss of men and started condemning the â€Å"awkward, inefficient, or ill-directed movements of men". This started in late nineteenth and early twentieth century. He transformed the old management system into new scientific management. Taylor actually wanted to increase the productivity of the organization through mutual understanding of trust between the managers, management and workers. Taylor suggested few techniques to improve the productivity and increasing the trust level. He suggested that 1) The rewards should be given to workers for high productivity. 2) Focus on the elimination of anxiety and stress from the work place. 3) Proper training and guidance should be provided to develop the capabilities in workers, 4) Eliminating the typical concept of â€Å"boss†, will increase the efficient and effectiveness at work? Further, Taylor also developed four principles for improving the productivity in scientific ma nagement theory that are 1) rule of thumb 2) scientific selection of the workers 3) Labor and management’s co-operation rather than the conflicts 4) Training and development of workers scientifically. However Taylor focused on breaking of a job into its parts, timeline for each part should be configured, and then reengineering the parts in a way that it minimizes the time and

Menu Essay Example | Topics and Well Written Essays - 250 words

Menu - Essay Example Immediately add the eggs, Gruyere, and Parmesan and pulse until the eggs are incorporated and the dough is smooth and thick. Spoon the mixture into a pastry bag fitted with a large plain round tip. Pipe in mounds 1 1/4 inches wide and 3/4-inch high onto the baking sheets. With a wet finger, lightly press down the swirl at the top of each puff. (You can also use 2 spoons to scoop out the mixture and shape the puffs with damp fingers.) Brush the top of each puff lightly with egg wash and sprinkle with a pinch of Gruyere. Bake for 15 minutes, or until golden brown outside but still soft inside. Place in a large kettle of scalding water and boil for 45 minutes. Remove and chill in cold water. Pull out all the hairs - these will have been loosened by the boiling and should come out easily (like plucking a duck). Wash thoroughly until no hairs remain. Place the nose in a kettle and cover with fresh water. Add onion, garlic, spices and vinegar. Bring to a boil, then reduce heat and simmer until the meat is tender. Let cool overnight in the liquid. When cool, take the meat out of the broth, and remove and discard the bones and the cartilage. You will have two kinds of meat, white meat from the bulb of the nose, and thin strips of dark meat from along the bones and jowls. Slice the meat thinly and alternate layers of white and dark meat in a loaf pan. Reheat the broth to boiling, then pour the broth over the meat in the loaf pan. Let cool until jelly has set. Slice and serve cold. 2 Combine the chocolate, butter, and espresso in the top of a double boiler over hot, but not simmering, water, stirring frequently until smooth. Remove from the heat and let cool until the chocolate is just slightly warmer than body temperature. To test, dab some chocolate on your bottom lip. It should feel warm. If it is too cool, the mixture will seize when the other ingredients are added. 4 When the chocolate has reached the proper temperature,

Supply Chain Strategy Essay Example for Free

Supply Chain Strategy Essay What recent changes have caused supply chain management to gain importance? 2. With so much productive capacity and room for expansion in the United States, why would a company based in the United States choose to purchase items from foreign firm? Discuss the pros and cons. The use of foreign firms can provide a U. S. firm more alternatives in selecting a supplier. The pros are more choices, potentially reduced costs in the areas of materials, transportation, production, and distribution, and potentially moving closer to a foreign market. The cons are the distance is generally increased, communications problems are increased due to distance, culture, and technology. There may be problems with customs, government regulations, political stability, etc. 3. Describe the differences between functional and innovative products. Functional products are staples that people buy in a wide range of retail outlets. Typically, they do not change much over time, have low profit margins, stable predictable demand and long life cycles. Innovative products, on the other hand, give customers additional reasons to buy. Fashionable clothes and personal computers are examples of innovative products. Innovative products have short life cycles, high profit margins, and volatile demand. 4. What are characteristics of efficient, responsive, risk-hedging and agile supply chains? Can a supply chain be both efficient and responsive? Risk-hedging and Agile? Why or Why not? As a supplier, which factors would you consider about a buyer (your potential customer) to be important in setting up a long-term relationship? The financial stability and credit worthiness of the company is of primary importance. The reputation of the company visavis their supplier is also very important. For example, is this a company that is fair with its suppliers and honors its payables in a timely fashion? Is the technological match between supplier and customer sufficient? Will delivery schedules and quantities be stable, facilitating smooth operations? For the value density example in Exhibit 10. 9, what would the effect be if a competing firm offers you a similar service for 10 percent less than Federal Express’s rates? 7. What are the advantages of using the postponement strategy? Process postponement delays the process step that differentiate the product to as late in the supply chain as possible. The advantages of this approach are that lower levels of inventory, and fewer models are needed to match customer requirements. This results in higher levels of customer satisfaction at a lower cost.

Perception of Leadership and Followership

Perception of Leadership and Followership Leadership and followership have the same coin but different face that we live by from the moment were born. Being on one of them does not mean youre just on that side, you can be a follower with your employer and a leader in your personal life. Both concepts have a broad meaning and cannot be adjusted into a short range or to a specific situation. Nevertheless, the relationship between them are endless and neither one of them can survive without the other. Moreover, whenever the leadership is setting an exemplary role, the follower will follow up exactly at the same level or at least the majority will do. Although, there is a correlation relationship between them, but it is always the leadership that has responsibilities to create reforms, expansions, and prosperity in the society. Furthermore, there is a very thin line between them and that line must be recognized by both side to ensure that commonsense balance is always present. For example, leaders sometimes forget to impose equ ality for all the followers under their responsibility due to the general system that has a limitation for them to spot that equality gap. Additionally, in this case, its better that a follower steps up to leadership and point out that gap and act accurately to close it by the right means. I feel that there is always a significant amount of useless area between leadership and followership that can be filled with enthusiasm, passion, and productivity. My family heritage and background have greatly influenced my perception of leadership in several ways. The experiences that you get in your early childhood days from the interactions that you have had with your family often re-emerges in your adult life interactions that you have with others including the interactions that you have in the business world. In that sense family becomes your first organization, and your family members become your first management team. It is your family life and upbringing that affects the ways in which the leaders respond to the pressures and the way they behave with their team members (Barry, 2015). My family upbringing was such that where I had many siblings and had many relatives with whom I used to interact quite often, which I believe has helped me develop my communication skills which I use as a leader also. The first time I realized my leadership potential was when I managed to finish my first big project in work much before the deadline and that project was a big deal to company performance and efficiency. Furthermore, the project was to transform hundreds of thousand paper data to electronic data which I was able to do with four members team and the timeline was two years and we finished it in just one year. As a result of my initiative, everything got sorted. There I was able to realize my leadership/influential skills. Values play a very crucial role in our lives. Besides taking decisions as per your values and philosophies, recognizing and understanding a given situation at your work place with respect to the values and philosophies of other team members is also very important. When an individual is able to establish a link between his or her own personal values with those of his or her employer, then a powerful connection is created (Anonson, 2014). Teaching can also be a way to shape the beliefs of a person regarding leadership. For example, The teacher who I can say had changed my life, and my views of leadership were my English teacher. When I was in 6th grade, there was a student in my class who was quite shy to speak up in the class, but Mr. Sultan (English teacher) showed faith in him and always encouraged him to speak even if he was wrong. And later on, that student became a very good spokesperson even at college level. At present concerning my professional work, I look up to my manager who is one of the executive, Mr. Abdullah as my role model for the most respective leader. The qualities that I admire about him are that he focusses on strength. He not just focusses on his own strengths or the strengths of the company, but also makes efforts to improve the strength of others in the organization as well. Besides this, he has got the qualities of trust. As an HR professional or line manager, HR Competencies will help me to become a better leader or follower and enhanced my team effectiveness. Earlier, before the development of leadership competencies, leadership was thought to be a task to be done only at the top level in the organization. Only the senior level people in the organization were able to take decisions and influence all in the organization (Shanafelt, 2015). Although, by the advancement of leadership competencies, it is now becoming an essential part in every level of the organization and not just by the upper-management. Leadership function that I was able to demonstrate while working with my team was being polite and friendly with my team members, and that gave me the advantage to have a better communication with them and to be opened to sort any misunderstanding that might happen. The Challenges and problems that I faced in my leadership were that though I was liked among my team members but due to this informal bonding, I lacked that respect that as a leader I should be getting and also people used to take my orders for granted. But as soon as I face this problem, I started working on the ways to overcome it. And for that purpose, I rather than encouraging informal communications, started taking up weekly formal meetings with all members. Race and gender also sometimes have an impact on leadership. When a female is given the position of a leader, many male team members refuse to follow her ideas. Also sometimes people do not cooperate with their leader on the basis of his nationality or caste. Ethics play a very important role in leadership. Sometimes leadership places employees in compromising positions which can result in dissatisfaction and thus can completely tarnish an organizations image and mission. Thus, leaders must take ethically correct decisions in the organization. On taking my self-assessment test, I got 37 points as a leader, which shows that I am a team player who enjoys working in the team. After my own experiences of working as a leader and a follower, I have noticed two dimensions of followership which are competency and relationship. Competency requires working well with others by analyzing them as a source of work. Whereas relationship requires building trust and improving communications. Whereas as a good leader, your strengths are being patient, confident, a role model and being polite to all. Using these strengths when your performing the leadership or followership role will enhance the reliability of your role and your part of any team. After assessing my own experiences, I can say that followers can either make or break the leaders by influencing the way the goals are achieved. Being a good follower means you take responsibility to back your leadership with any assistance they need to be on the right path and most importantly remained them if they lose their tracks. By being a good follower, you dont really have to be a sheep and follow blindly to the leader, but rather you must develop those skills of a good follower which will help you become a better leader (Derler, 2014). Thus, my future goals would include becoming a good follower to become a role model when following ethical leadership when it comes to being a leader. Thus, it can finally be concluded that followership is nothing but the mirror image of leadership and becoming a good follower will not let you down rather will help you in becoming a great leader. Also, leadership is inefficient without good followers. References Barry, T. R. (2015). Top 10 qualities of a project manager Anonson, J., Walker, M. E., Arries, E., Maposa, S., Telford, P., Berry, L. (2014). Qualities of exemplary nurse leaders: perspectives of frontline nurses. Journal of nursing management, 22(1), 127-136. Shanafelt, T. D., Gorringe, G., Menaker, R., Storz, K. A., Reeves, D., Buskirk, S. J., Swensen, S. J. (2015, April). Impact of organizational leadership on physician burnout and satisfaction. In Mayo Clinic Proceedings (Vol. 90, No. 4, pp. 432-440). Elsevier. D. (2015). Leaders in the Shadows: The Leadership Qualities of Municipal Chief Administrative Siegel, Officers. University of Toronto Press. Black, S. A. (2015). Qualities of effective leadership in higher education. Open Journal of Leadership, 4(02), 54. Derler, A., Weibler, J. (2014). The ideal employee: context and leaders implicit follower theories. Leadership Organization Development Journal, 35(5), 386-409. Bligh, M. C., Kohles, J. C. (2015). Approaching leadership with a follower focus.

Role of Women in the Epic of Beowulf and Anglo-Saxon Society Essay

Role of Women in Beowulf and Anglo-Saxon Society  Ã‚  Ã‚  Ã‚  Ã‚   Beowulf, the hero of Anglo-Saxon epic, had many adventures, and many companions and fellow-warriors are mentioned throughout his story. Some of them seem noble and courageous, truly living up to the standards of their culture; some seem cowardly. But all have gained immortality in the words, many times transcribed and translated, of the famous epic. However, the women of the time are rarely mentioned in Beowulf. Still, even from those few women who are mentioned and from other documents of the era, it is possible to see the position of women in of Anglo-Saxon society. In many cases, they enjoyed more rights than women in later Medieval cultures. Both Wealhtheow, Hrothgar's queen, and Hygd, Hygelac's queen, apparently held power in their courts. Wealhtheow's actions in rewarding Beowulf after his battles show the queen's role and position as hostess. She awards him "two arm ornament... ... impact on today's life, goes far toward dispelling notions that the Anglo-Saxons restricted the freedom of women in favor of a purely male-oriented, hero-worshiping society. Works Cited Beowulf and Other Old English Poems. Trans. Constance B. Hieatt. New York; Odyssey Press, 1967. Gies, Frances and Joseph. Marriage and Family in the Middle Ages. New York; Harper and Row, 1987. Page, R.I. Life in Anglo-Saxon England. New York; G.P. Putnam's Sons, 1970.

The Oppression of Females in Advertising :: essays papers

Our society is a complex collection of institutions, status, roles, values, and norms, and the best way to understand and learn about them is through the use of cultural artifacts. These can be anything from music to art to literature, or as in the example of this discussion, the modern day creation of advertisement as seen in women's magazines. As Homo Sapiens moved from the hunter - gatherer way of life to industrial society , it was necessary to construct a framework for living so that such a concentrated number of people could exist together. This framework as come to consist of a myriad of expectations based on values and norms in the form of roles status and institutions. Desirable behavior is sought by people throughout the country based on how one is brought up and the expectations one is bombarded with on a daily basis. These expectations are reflected in every part of our culture and are used by people so as to know how to act in any given situation. The main examples are: the family, education, health and medicine, religion, and the law. I have found that certain mediums reflect the expected roles in these institutions better than others. I originally focused on gender roles as a depiction of stereotypical behavior as reflected by advertising especially the portrayal of women, but I discovered that there were other stereotypes being perpetuated as well that were just as institutionalized if not just simply less noticed or studied. Therefore, although this argument will focus on the depiction of females and the female role in advertising. It will also mention the general use of American values , norms, and institutions to influence consumers. An institution is defined as "a stable cluster of values, norms, status, roles, and groups that develop around a basic social need" with a status being a person's position in society and a role being the behavior expected from that, and a value being a socially shared idea of what is good, right, and desirable and a norm being the behavior expected from those ideas. When people begin to form certain expectations in life there begin to be formed stereotypes. People recognize them universally and use them to form opinions and act or not act in a certain way when they are confronted with a situation or person.

Boeing 7 Essay

A reverse of this trend is crucial to reach some of the projected sales numbers that Boeing is counting on. There are several factors to these lower numbers. A decrease in business travel has occurred due to cost and the advance of conferencing technologies. And lastly, the weak economy has vacationers thinking of local destinations instead of traveling abroad. Market Share Boeing’s fiercest competitor is Airbus. It is crucial that the new 7E7 delivers on its promise of lower operating cost. This will help command a larger share of the market. This becomes even more important if the economy doesn’t recover as quickly as we hope. The other aspect of the 7E7’s success is the engineering of an expandable wing. Adding this versatility will give the 7E7 owner more options for travel routes. Sensitivity Analysis The following is the sensitivity analysis of the Boeing project which gives optimistic and pessimistic estimates for the underlying variables of volume and cost of sales. The purpose of the sensitivity analysis is to express cash flows in terms of the variables of this project. Boeing had to determine what the decide what the underlying variables were which in this case happen to be development costs and the per-copy costs to build the 7E7. For the project to increase shareholder wealth, Boeing would have to sell at least 2,500 aircraft over a 20-year period. Airbus is a close competitor. They will be coming to market with a new A380. If Boeing falls behind regarding innovation, they may lose their market share. For Boeing to have a future in the aviation industry, they must take the risk to develop this new place. With the economy so volatile, airlines will be looking for options that reduce their operational costs. The 7E7 will do this. The success of the expandable wing will give the plane attractive versatility. Fuel prices are also on the rise, this is yet another reason why a plane with a lower operating cost will succeed in the future The equity market risk premium should equal the excess return expected by investors on the market portfolio. In this case it was calculated to be 7. 14%. The weighted average cost of capital (WACC) was calculated to be 15. 443%. For the project to increase shareholder wealth, the IRR of the project should at least equal the WACC. For this to happen Boeing would have to sell at least 2500 airliners in a 20-year period.

The Scramble for Africa

What were the major historical factors explaining ‘the scramble for Africa’? The scramble for Africa has aptly been described as the golden period of European expansionism in the 19th century. It was an age in which the continents of Africa, Asia and Middle Eastern states were brought under the control of European powers following the Berlin Conference of 1884-1885. Eric Hobsbawm, one of the leading authorities on European imperialism, described the period as â€Å"the Age of Empire not only because it developed a new kind of imperialism, but also a much more old-fashioned reason†¦Ã¢â‚¬  referred to here as the age of â€Å"emperors† (1987: 56). It was essentially a period in which a handful of European powers (Great Britain, France, Portugal, Belgium, Netherlands, Italy, Japan, Germany etc), having emerged economically strong following rapid industrialisation, set out to pursue radical national interests overseas. The scramble for Africa began at a time when the benefit of industrial revolution gave rise to unprecedented expansion in the production of goods and services, which needed to be exported to outlandish markets. For, the partition and the haggling that went it did not come out of the blue. It was orchestrated by a combination of factors and conditions under which European powers faced in their metropolitan countries at the time. Having lost their North and South American colonies, Australasia and the Pacific rim interests at the turn of the century, the European powers turned their searchlight to Africa, Asia and the Middle East for new markets – consolidating previously held trading posts and sea route communications and grabbing new territories along the way – hence the scramble for Africa. The partition has broadly been described as one of the most turning points in the history of the relationships between the â€Å"Haves† – industrialised European powers versus the â€Å"Have-nots† – tropical Africa and the countries of Asia and the middle East (Padmore, 1972: 7). In his most eloquent work on the subject of partition – Africa and the World Peace (1972: 162), Padmore argues that â€Å"†¦ colonial policy is the offspring of industrial policy for rich States in which capital is abundant and is rapidly accumulating, in which the manufacturing system is continually growing and characterising, if not most numerous, at least the most alert and energetic part of the population that works with its hands, in which the countryside is obliged to industrialise itself, in order to maintain itself, in such States exportation is an ssential factor of public property †¦Ã¢â‚¬  Still, Jules Ferry, â€Å"who can fittingly be described as the father of French Imperialism, whilst addressing the Chamber of Deputies in 1885, summed up the need for colonies as follows: Is it not clear that the great States of modern Europe, the moment their industrial power is found, are confronted with an immense and difficult problem, which is the basis of indust rial life, the very condition of existence – the question of markets? †¦ Can we say that this colonial policy is a luxury for modern nations? Not at all †¦ this policy is for all of us, a necessity, the market itself† (p. 161). Ferry’s encapsulation of what the partition of Africa meant for the French and his fellow European powers are quite instructive here. Similarly, continental echoes of the partition policy were heard in ascending order. In the Island of Great Britain, Mr Joseph Chamberlain, â€Å"the radical mayor of Birmingham and a great advocate of liberal ideals, who later deserted the Liberals and became one of the most ardent champions of Toryism †¦Ã¢â‚¬ , accepted that â€Å"a forward policy of colonial expansion in Africa was now the order of the day†. He stated that: â€Å"it is the duty of the State to foster the trade and obtain markets for its manufactures† (p. 164). In Germany, Bismarck, who initially opposed colonial expansion, later became its advocate. Addressing the Reichstag in 1885, he declared that: â€Å"The goal of Germany’s foreign policy was to be economically independent. â€Å"Colonies†, he said, â€Å"would provide new markets for German industries, the expansion of trade, and new field for German activity, civilisation and capital †¦ Consider what it would mean if part of the cotton and coffee which we must export could be grown in German territory overseas. Would that not bring an increase in national wealth†? (Padmore, pp. 164 -165), he queried. H. L. Wessseling, in his Divide and Rule: The Partition for Africa (1996: 366), whilst analysing Hobson’s classic work on Imperialism: A Study, argued that the historical interpretation of the partition was based on â€Å"imperialism as a consequence of capitalism† and therefore, â€Å"primarily a struggle for profitable markets of investment†. He acknowledged the seminal work of John Gallagher and Ronald Robinson in their Africa and the Victorians – stating that it was not until the 1960s that a new approach began to be adopted by the leading discussants of the partition policy. He opined that aside from the economic motives upon which the partition was based, there was indeed, consideration of strategic and political motives as well, noting that early writers overlooked this fact. He summed up the views of the British political establishment thus: â€Å"The British policy makers were not so much concerned with Africa itself as with safeguarding British interests in Asia; the motive behind late Victorian strategy in Africa was to protect the all important stakes in India and the East† (p. 366). This view becomes clearer when juxtaposed with Hargreaves’ Chapter 3 in Decolonisation in Africa whilst discussing the logistics of the Second World War. He stressed that â€Å"the protection of African supply route was a crucial contribution to the Middle East war†, adding that â€Å"extraordinary efforts were made to develop the African Line of Communications by which bulky supplies were moved to Congo river, across to Juba in the Southern Sudan, and thence to Egypt† (Hargreaves, 1988: 54). Surely, there was no question of the strategic and commercial importance of Africa, Asia and the Middle Eastern territories to the Allied Powers prior to, and during, the Second World War. As M. E. Chamberlain succinctly attested here: â€Å"the possession of an empire came to be regarded as a kind of badge of great power status, important for prestige, irrespective of whether it was worth while economically† (Chamberlain, 1985: p. 3). Whilst huge merit pertains in this argument, there’s no doubt as Wesseling puts it: the policy of imperialism in Africa, Asia and the Middle East â€Å"differed from country to country, from period to period, and from place to place† (Wesseling, 1996, p. 366). The argument makes it clear: â€Å"economic motives such as the protection and encouragement of trade and industry did indeed play a part †¦ so also did such financial motives as safeguarding of loans and investments, such political motives as strategic advantage, national ambition, electoral appeal, such as ideological motives as bearing the white man’s burden, and many more† (p. 366). The method used by the European powers in gaining foothold into the African territories was generally regarded as underhand. Africans and Indians, it was assumed, only began to exist at the point they were â€Å"discovered† (Liebenow, 1986: p. 4). Accordingly, â€Å"treaties of friendship negotiated †¦ with local political personages, or evidence of conversations alone, became converted in the 19th century diplomatic scramble into European deeds of ownership to the land, the people, and all their resources† (p. 14). Following the inordinate ways used in slicing up African territories , the Berlin conference recognised Leopold’s claims over Congo, and the various spheres of British, French and German influence in the East, West and South Africa respectively. The period following the conference was marked by the rapid annexation of the territories involved. In order to consolidate their positions, England, France and Germany first resorted to the use of Chartered Companies (British and German East Africa Companies, the Niger Company of West Africa, and the South Africa Company), joint stock organisations with tremendous financial resources at their disposal, backed by the armed forces of their respective States. These monopoly concerns were the ones which laid the basis of government in the territories which were later officially declared as colonies and protectorates (Padmore, p. 168 – 169). Germany, although a late entry in the colonial race, acquired German East Africa (then known as Tanganyika) in 1844, South West Africa in 1885, Cameroons and Togoland in 1885 (P. 168 – 169). â€Å"By the time the process of carving up Africa was completed, England and France had emerged as the biggest shareholders of the continent†. â€Å"England acquired the colonies of Gambia, Sierra Leone, Gold Coast (now Ghana), Nigeria on the West Coast, British Somaliland, Kenya, Uganda, Tanzania, Zambia, Zimbabwe (then Southern Rhodesia), Swaziland and Basutoland, and the Union of South Africa. France, on the other hand, got most North Africa countries of Algeria, Tunisia and Morocco. On the West Coast of Africa, it secured Senegal and its hinterland, forming Equatorial Africa and the Congo, Dahomey, Ivory Coast and the large Island of Madagascar. After the World War, the German West and East African colonies were later divided between the British Empire and France following the Paris conference† (p. 169), using the League of Nations to consolidate their booties. Portugal, one of the oldest colonial Powers in the world got Angola and Guinea on the West Coast, Mozambique on the East, and the cocoa island of Sao Tome and Principe in the Gulf of Guinea† (p. 169). â€Å"Italy, having met military disaster in her early imperialist attempt at Abyssinia in 1896, as well as diplomatic defeat by France over Tunisia, acquired Tripoli in the north, Eritrea on the East Coast and Italian Somaliland on the Indian Ocean† (p. 169). It has commonly been argued that the ap proach used by the European powers in running their territories exacerbated local anger against colonial rule. Many ethnic groups with little in common were lumped together, thus creating confusion and rivalries, making the present day African countries extremely difficult to govern. Whilst Britain employed a mixture of direct (India) and indirect rule (Africa and others), using recognised local people to govern – the French and others tended to adopt a more direct approach. For example, the French and the Portuguese believed in the policy of integration or assimilation. This policy extended French citizenship to trained Africans whilst providing a token of autonomy to local representatives who, subsequently, were co-opted into French Parliament. While Africa provided the best example for studying the development and expansion of European Imperialisms in their quest for markets, sources of raw materials and spheres for investing capital, this overseas projection of European capitalism was not confined to the Dark Continent. Indeed, scramble incursions were made into Asia and other parts of New World. The chief amongst these was India, often referred to as the â€Å"Jewel in the Crown† because â€Å"it differed from all other colonies of occupation in its vastly greater size and population, reaching 200 millions in 1860s† (Fieldhouse, 1965/6: 271). According to Fieldhouse, â€Å"India provided Britain with political and military power†Ã¢â‚¬ ¦ therefore, â€Å"its resources were harnessed to support a great military empire before the British arrived† (Fieldhouse: p. 271). In summing up, therefore, it has to be argued that the â€Å"European domination of Africa, Asia and the Middle East has been one of the most significant phenomena of the 19th century period called â€Å"the modern age† (Liebenow, 1986: 13). The technological superiority of the European powers and the age of industrial revolution led the West in believing that they were destined, as a matter of right, to govern people elsewhere on the globe. Regrettably, colonisation was â€Å"unable to shape African economic, social and political conditions to more than a very limited extent† (Wesseling, 1996: 372). â€Å"In economic or social respects, colonisation brought nothing essentially new †¦ but only led to the acceleration of social and economic process of modernisation† (p. 372). This led to the integration of Africa and the rest of the New World into the capitalist economy. If we have to look for any tangible benefit of colonialism, this has to be seen in the context of the multiplicity of states that sprung up in Africa with concomitant ethnic conflicts and political instabilities. The false notion of sovereignties accorded to African states and recognised by the United Nations, clearly shows that majority of these states are weak and unable to clear democratic legitimacies in their various territories.

Dishonest salespeople and gossip Essay

No matter what culture one belongs, common observation shows that women â€Å"gossip† while men â€Å"talk shop†; women are â€Å"bossy† and men are â€Å"firm† (Women’s Language, 2005). However, even if common observation shows that women talk more than men, research findings prove otherwise. In fact, findings would even show that â€Å"men have been shown to talk more than women in settings as diverse as staff meetings, television panel discussions and husband-and-wife pairs in spontaneous conversation† (Women’s Language, 2005). As men tend to talk about â€Å"male things† like politics, sports, cars, women talk about the â€Å"women things† such as child-rearing, household chores and some personal relationships. According to Pamela Fishman’s research studies, women are relatively weak in interactive situations because they tend to â€Å"exploit questions and answers in order to force a response and keep the conversation going† (Women’s Language, 2005). Therefore, â€Å"gossiping† is common and universal. People always love to hear something about the other person who is not on the scene of conversation. What about dishonest salespeople? (rhetorical question) Are these people more disgusting than those who gossip? Looking at the gravity of damage which one causes more harm?   Looking at the gravity between the results, which causes greater damage? Looking at the gravity of the situation, it seems that gossiping is the most destructive trait here. (parallelism). But of course, people will not discount the fact that dishonesty in sales is also a bad trait. For one, not all incidents of dishonesty are detected and not all detected cases are reported. Therefore any fraud statistic is an estimate. Nevertheless, the statistics make it clear that dishonesty in salespeople occurs frequently, and no organization is immune (Peterson & Zikmund, 2004). The costs of fraud and dishonesty will continue to rise unless auditors, management, and the general public become more proactive in learning about the types of fraud, the perpetrators and valuable fraud prevention and detection techniques (Bezanis, 2002). It is important to be able to know the difference between the real and spurious products in order to maintain the good image of that company. Fraud is conventionally defined as â€Å"intentional deception, deceitful pretenses, or deliberate trickery to gain an advantage† (Hanlin, 2004). Fraud encompasses an array of irregularities and illegal acts characterized by intentional deception. Every fraud involves three elements: (1) theft act, (2) concealment, and (3) conversion (Albrecht & Albrecht, 2001). For instance, buyers who get the misfortune of buying fake Gucci bags are able to identify the fake product because of the flimsy hardware, cheap leather and misspelled logos. The fake bags are so like the originals that it is hard to spot them quickly. There are businessmen who are able to copy the smart way. High-end label bags cost around $500 to over $1,000. Anything less than these price ranges are fake. Buyers are also encouraged to read the fine print so that they do not buy those that says, â€Å"Designer Inspired.† The numerous issues which plague the business sector with allegations and prosecution of unethical conduct easily fall under either the definition of fraud or unfair and deceptive practices. Or if the issue does not fit with the two terms, it can fall under unethical business conduct. The unethical conduct is a catchall phrase that includes fraud and unfair practice and other aspects beyond the scope of the two terms. In the same manner, gossip is also an unethical business indulged by people regarding other people. However, gossip causes hurt. It can hurt people and damage lives. Thus, gossip, depending on the seriousness can be extremely dangerous too. It can damage a person’s reputation and image. In the long run, gossip seems to be more damaging than dishonest salespeople because one can always return a fake product. But the destruction one causes in gossip spreads like wildfire and cannot be restored. As defined, ethical business conduct is doing something that is not required by law, contract, or other obligation and which is a positive contribution to society. Therefore, a contrary conduct is unethical. This means doing something which is prohibited by law, contract, or other obligation or which has a negative contribution to society. Contribution to society is now part of the definition because ethical business conduct has evolved into what is termed as corporate and social responsibility. An act of the company has a vast effect on the society as a whole. In the course of human interactions, there are many situations in which it is difficult to make a decision because values come into conflict. It is essential that an individual or an organization engage in values clarification to develop a personal decision-making process that fosters ethical behavior.

Svrwvrev

LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO. – 10800037 ROLL NO- RB1R07B02 B. TECH BIOTECH. (8th sem. ) DATED- . 17-05-2012 CERTIFICATECertified that this project entitled â€Å"anti microbial activity of different types of honey † submitted by MOHIT KUMAR , students of biotechnology Department, Lovely Professioal University, Phagwara Punjab in the partial fulfillment of the requirement for the award of Bachelors of Technology (biotechnology) Degree of LPU, is a record of student’s own study carried under my supervision & guidance. This report has not been submitted to any other university or institution for the award of any degree.Date: 17/5/2012 Name of Project Guide Dr. Akshay Garg DECLARATION I, MOHIT KUMAR, student of B. Tech Biotechnology under Department of Biotechnology of Lovely Professional Unive rersity, Punjab, hereby declare that all the information furnished in this dissertation / capstone project report is based on my own intensive research and is genuine. This dissertation / report does not, to the best of my knowledge, contain part of my work which has been submitted for the award of my degree either of this niversity or any other university without proper citation. Date – 17/5/2012 Investigator- MOHIT KUMAR Regd. No. 10800037 Acknowledgement Any attempt at any level can't be satisfactorily completed without the support and guidance of learned people in my capstone projectâ€Å"Antimicrobial activity of different types of honey†.I would like to express my immense gratitude to my guide Dr. Akshay Garg for his constant support and motivation that has encouraged me to come up with this project. I also would like to thanks my group member who helped me in my project. MOHIT KUMAR ABSTRACT Honey is a traditional topical treatment for infected wounds. It can be effective on antibiotic-resistant strains of bacteria. Several local brands of honey(Dabur) collected from different sources is used in this study. Also the honey is collected from various apiaries from Pathankot(Punjab) and Saharanpur (Up).The bacterial culture were taken from the laboratory of the Lovely professional University and was revived in nutrient broth media and then sub-cultured in nutrient agar media. The method used to test this antibiotic sensitivity of honey was done with the help of Kirby Bauer method . Marked variations were observed in the antibacterial activity of these honey samples. Bacterial species used were Bacillus subtilis ,E. coli. , S. aureus and Burkholderia spp. The zone of inhibition produced against different bacteria using honey of different concentration are as follows: E. oli (pathankot sample): 100% = 2. 6cm, 75%= 1. 9cm, 50%= 1. 7cm,and 25%= 1. 2cm; E. coli (dabur sample): 100% = 2. 2cm, 75%= 1. 6cm, 50%= 1. 3cm, and 25%= 1. 1cm; E. coli (sahar anpur sample): 100% = 2. 8cm, 75%= 2. 4cm, 50%= 2. 2cm, and 25%= 1. 7cm; B. subtilis (pathankot): 100% = 3. 0cm, 75%= 2. 7cm, 50%= 2. 7cm, and 25%= 2. 5cm; B. subtilis (dabur): 100% = 2. 7cm, 75%= 2. 5cm, 50%= 2. 4cm, and 25%= 2. 2cm; B. subtilis (saharanpur):100% = 3. 2cm, 75%= 2. 7cm, 50%= 2. 3cm, and 25%= 1. 5cm; Burkholderia spp. (pathankot): 100% = 2. 1cm, 75%= 1. 9cm, 50%= 1. 6cm, and 25%= 1. 4cm; Burkholderia spp. dabur): 100% = 2. 4cm, 75%= 1. 7cm, 50%= 1. 5cm, and 25%=1. 2cm; Burkholderia spp. (saharanpur):100% = 2. 5cm, 75%= 1. 9cm, 50%= 1. 7cm, and 25%=1. 4cm; S. aureus (pathankot):100%=2. 1cm, 75%= 1. 7cm, 50%= 1. 4cm, and 25%=1. 2cm; S. aureus (dabur):100% =3. 0cm, 75%= 2. 7cm, 50%= 2. 2cm, and 25%=1. 8cm; S. aureus (saharanpur): 100% = 2. 9cm, 75%= 2. 5cm, 50%= 2. 0cm, and 25%=1. 6cm; Zones of inhibition of different treatment groups were measured by agar-well-diffusion assay and compared with control. The comparison of honey sample with distilled water control had pro ved it significant.CONTENTS PAGE 1. INTRODUCTION 7 1. 1) Honey as antibacterial agent 7 1. OBJECTIVE 9 2. LITERATURE REVIEWS 10 3. 1 TYPES OF HONEY 10 3. 2 COMPONENTS OF HONEY 11 3. MATERIALS AND METHODS 15 4. MATERIAL 15 4. 2 METHOD 16 4. RESULTS AND DISCUSSION 19 5. REFERENCES 32 1. ) INTRODUCTION Honey is a sweet food made by bees using nectar from flowers. The Honey is a sweet, viscous fluid produced by bees from the collection of nectar, primarily from flowers. It is considered to be a natural syrup. The Nectar is gathered by the bees and is slowly transformed into honey, through a long rocess involving the addition of enzymes and the gradual reduction of moisture. Honey is a rich source of carbohydrates mainly Fructose and Glucose. The chemical composition of honey varies depending on the plant source, season and production methods. Therefore the Colour, Concentration and Compounds vary depending on the floral sources. Other compounds which can be found in Honey include Pr oteins and acids such as Gluconic Acid (C6H11O7, also known as 2,3,4,5,6- pentahydroxyhexanoic Acid), Minerals and Anti-Oxidants such as Hydrogen Peroxide (H202) and Vitamins (B6 and B12), (BD.Yates et. al. 1996). Honey has a low pH and a low moisture content, which is usually on average about 17 percent. The Gluconic Acid in honey is produced when bees secrete Glucose Oxidase, while processing the nectar, this give honey a low pH. There are many varieties of honey from around the world which come in three main types which are liquid, whipped and comb. Several local brands of honey(Dabur) collected from different sources is used in this study. Also the honey is collected from various apiaries from Pathankot(Punjab) and Saharanpur (Up) .Each of these honey’s were chosen because they are organic and readily available in health food stores 1. 1) The Antibacterial Effects of Honey The honeys are shown to have antibacterial properties, in particular Manuka honey. Manuka Honey has had extensive research done on it. It has been shown in many studies that Manuka Honey has antimicrobial effects (Barret J. , et. al, 2005; Coumbes A. L. , et. al. 2004; Mundo, M A. 2004). The Mechanism of Antibacterial Properties Honey has many natural properties which enable it to inhibit bacteria.These properties include, a low pH which is in the range of pH 3. 2 to 4. 5, approximately 3. 9 which is due to its content of acidic compounds mainly Gluconic acid as stated above. A low pH is inhibitory to most bacteria. Since most bacteria live in environments around pH 7, the pH of honey could inhibit the bacteria (Barrett, J et. al. al 2005). This is because pH affects the way large proteins such as enzymes work. Which causes the shape of enzymes to change, which then alters the overall charge, this causes the protein to denature.Honey contains small amounts of Hydrogen peroxide (H202) this varies depending on the honey, it is produced as a result of the enzyme glucose oxidase activ ity in producing Gluconic acid (Mundo, MA. et. al. 2004). C6H12O6 + H2O + O2 > C6H12O7 + H2O2 (glucose oxidase reaction) When honey is used topically (as, for example, a wound dressing), hydrogen peroxide is produced by dilution of the honey with body fluids. As a result, hydrogen peroxide is released slowly and acts as an antiseptic. Hydrogen peroxide is a powerful oxidising agent (Free Radical) which has the ability to damage cells.In an aqueous solution hydrogen peroxide acts like an acid and can oxidise a variety of compounds, by accepting free unpaired electrons. This allows the formation of other free radicals, which then causes a cascade effect. Therefore altering biological structures and therefore damaging cells. Honey is primarily a saturated mixture of two monosaccharide, with a low water activity; most of the water molecules are associated with the sugars and few remain available for microorganisms, so it is a poor environment for their growth.If water is mixed with hone y, it loses its low water activity, and therefore no longer possesses this antimicrobial property. Honey consists of various constituents such as water, carbohydrates, proteins, vitamins, amino acid, energy and minerals. Besides the major ones, there must also be several minor constituents in honey, which may be playing a key role in determining the antimicrobial behaviour of honey. In the past, antimicrobial activity of honey had been reported only by using aqueous solution of honey.It is said that honey possesses antibacterial property but it is not clear whether it is the bulk honey or some fraction of it. The antibacterial properties of honey speed up the growth of new tissue to heal the wound. The bactericidal effect of honey is reported to be dependent on concentration of honey used and the nature of the bacteria Considering the fact that their might be some specific constituents which may be contributing to the antimicrobial behaviour, it was decided to carryout the studies u sing different solvents.The present study therefore deals with the constituents in the different solvents followed by evaluation of extract for their antimicrobial behaviour against certain species of bacteria. 2) OBJECTIVE Based on the above facts, the present study was defined with following objective: 1) To check the antibacterial effect of different honeys on. a. Gram-positive Staphylococcus aureus, b. Gram-negative Escherichia coli, c. Gram-positive Bacillus subtilis, and d. Gram- negative Burkholderia capicium 2) To check the effect of honey on bacterial strains using different-different dilutions. . 100% honey sample b. 75% honey sample c. 50% honey sample d. 25% honey sample 3) To compare the effect of honey and control (Distilled water) on a. Gram-positive Staphylococcus aureus, b. Gram-negative Escherichia coli, c. Gram-positive Bacillus subtilis, and d. Gram- negative Burkholderia capicium 3) LITERATURE REVIEWS Honey has been used with medicinal purposes since ancient tim es and there are reports of its topic use by Ayurvedic medicine, dating back to 2500 b. c. Hippocrates prescribed the use of honey for several indications, including treatment of wounds and gastritis.Its healing properties are mentioned in the Koran and in Bible. Honey is a part of traditional medicine for centuries. The antimicrobial activity of honey is due primarily to hydrogen peroxide produced enzymatically. However, in undiluted honey the acidity is also a significant antimicrobial factor. The pH, ranging from 3. 2 to 4. 5, is low enough to inhibit the development of pathogens, whose optimal pH range situates between 7. 2 and 7. 4. When applied to affected areas of tissue, acidity, osmotic effect and phytochemical factors decrease with dilution; however, the activity of hydrogen peroxide increases from 2. to 50 times. On these values, peroxide acts as an antiseptic, without causing cell damage. The composition of sugars in honeys, from different floral origins, inhibits the de velopment of various intestinal bacteria. All chemical and physical factors make the therapeutic properties of honey unique: fast decline of infections and healing of wounds, quick inflammation recovery, minimizing of wounds, stimulation of angiogenesis, as well as the development of epithelial and granular tissues. Honey to be used with topical medicinal purpose, certain requirements are needed, like being free f herbicides, pesticides, heavy metals and radioactive elements and sterilized to prevent secondary infections. 3. 1) TYPES OF HONEY – ON BASIS OF FLORAL SOURCE- Blended- Most commercially available honey is blended, meaning it is a mixture of two or more honeys differing in floral source, color, flavor, density or geographic origin. Polyfloral – Polyfloral honey, also known as wildflower honey, is derived from the nectar of many types of flowers. The taste may vary from year to year, and the aroma and the flavor can be more or less intense, depending on which bloomings are prevalent.Monofloral- Monofloral honey is made primarily from the nectar of one type of flower. Different monofloral honeys have a distinctive flavor and color because of differences between their principal nectar sources. To produce monofloral honey, beekeepers keep beehives in an area where the bees have access to only one type of flower. Honeydew honey- Instead of taking nectar, bees can take honeydew, the sweet secretions of aphids or other plant sap-sucking insects. Honeydew honey is very dark brown in color, with a rich fragrance of stewed fruit or fig jam, and is not sweet like nectar honeys. . 2) COMPOSITION OF HONEY Carbohydrates Unsurprisingly, these comprise the major portion of honey – about 82%. The carbohydrates present are the monosaccharide fructose (38. 2%) and glucose (31%); and disaccharides (~9%) sucrose, maltose, isomaltose, maltulose, turanose and kojibiose. There are also some oligosaccharides present (4. 2%), including erlose, theanderose and panose, formed from incomplete breakdown of the higher saccharides present in nectar and honeydew. Proteins and Amino Acids.Honey contains a number of enzymes, including invertase, which converts sucrose to glucose and fructose; amylase, which breaks starch down into smaller units; glucose oxidase, which converts glucose to gluconolactone, which in turn yields gluconic acid and hydrogen peroxide; catalase, which breaks down the peroxide formed by glucose oxidase to water and oxygen; and acid phosphorylase, which removes inorganic phosphate from organic phosphates. Honey also contains eighteen free amino acids, of which the most abundant is proline. Vitamins, Minerals and AntioxidantsHoney contains trace amounts of the B vitamins riboflavin, niacin, folic acid, pantothenic acid and vitamin B6. It also contains ascorbic acid (vitamin C), and the minerals calcium, iron, zinc, potassium, phosphorous, magnesium, selenium, chromium and manganese. The main group of antioxidants in hon ey are the flavonoids, of which one, pinocembrin, is unique to honey and bee propolis. Ascorbic acid, catalase and selenium are also antioxidants. Generally speaking, the darker the honey, the greater its antioxidising properties. Other compoundsHoney also contains organic acids such as acetic, butanoic, formic, citric, succinic, lactic, malic, pyroglutamic and gluconic acids, and a number of aromatic acids. The main acid present is gluconic acid, formed in the breakdown of glucose by glucose oxidase. Honey also contains hydroxymethylfurfural, a natural product of the breakdown of simple sugars below pH 5. According to studies done by Patricia E. Lusby et al Twelve of the 13 bacteria were inhibited by all honeys used in this study with only Serratia marcescens and the yeast Candida albicans not inhibited by the honeys.Little or no antibacterial activity was seen at honey concentrations 1%, with minimal inhibition at 5%. No honey was able to produce complete inhibition of bacterial g rowth. Although Medi honey and manuka had the overall best activity, the locally produced honeys had equivalent inhibitory activity for some, but not all, bacteria. He then postulated that honeys other than those commercially available as antibacterial honeys can have equivalent antibacterial activity. These newly identified antibacterial honeys may prove to be a valuable source of future therapeutic honeys. According to Peter C.Molan (2001), honey may be the â€Å"natural cure† for most bacterial infections that could replace man-made antibiotics. Researchers say that an enzyme in the honey turns into a tiny  amount hydrogen peroxide when combined with bodily fluids killing nearby bacteria. Honey also causes an increase in lymphocyte and  phagocyte activity (Cooper et al. , 2011). The honeys are shown to have antibacterial properties, in particular Manuka honey. Manuka Honey has had extensive research done on it. It has been shown in many studies that Manuka Honey has an timicrobial effects (Barret J. , et. al, 2005; Coumbes A. L. , et. al. 2004; Mundo, MA. 2004).In this study the antibacterial activity of the five honeys were compared, the Manuka honey was used as a positive control. The nonperoxide antibiotic activity is due to methylglyoxal (MGO) and an unidentified synergistic component. Most honeys contain very low levels of MGO, but manuka honey contains very high levels. The presence of the synergist in manuka honey more than doubles MGO antibacterial activity. In vitro antibacterial activity of raw and commercially available honey was tested against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella spp. nd Salmonella spp. ). Both types of honey showed antibacterial activity against test organisms with the zone of inhibition ranging from 8. 13 to 30. 85 mm, while E. coli, S. aureus, and Shigella spp. showed sensibility towards both types of Honey. Both types of honey showed no effects on Salmonella spp. The potency of honey at 100%concentration was found to be higher than all other concentrations tested. However, no effect was observed at concentration of 6. 25% v/v honey in the case of both samples(Patricia E. Lusby 2004).To study the antimicrobial activity of honey, 60 samples of various botanical origin were evaluated antimicrobial activities against 16 clinical pathogens and their respective reference strains. The bioassay applied for determining the antimicrobial effect employs the well-agar diffusion method and the estimation of minimum active dilution which produces a 1 mm diameter inhibition zone. Streptococcus pyogenes,Bacillus cereus and Bacillus subtilis were proven to be up to 60% more resistant than their equal reference strains thus emphasizing the variability in the antibacterial effect of honey and the need for further research (C.Voidarou 2010). Natural products, either as pure compounds or as standardized plant extracts, provid e unlimited opportunities for new drugs because of the ready availability of chemical diversity (Cos et al. , 2006). Honey and lemon-honey are traditional remedies in the Middle East and China and for many centuries and have been used in the treatment and prevention of the common cold and various upper respiratory tract infections (Molan, 1992; Zulma ; Lulat, 1989). Antibacterial activity of the honeys was assayed using standard well diffusion methods. oncluded that the sandal wood from different sources and mixture of of – and -santanols were highly effective against gram negative bacteria including Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae and as well as yeast Candida albicans. The present work gives strong evidence of antibacterial activities of honey, sandal oil and black pepper (Sheikh Ahmad 2002). In general, all types of honey have high sugar content as well as low water content and acidity, which prevent microbial growth.Osmotic effect, effect of pH and hydrogen peroxide are represented as an â€Å"inhibition† factor in honey (Postmes et al. , 1993). Most types of honey generate hydrogen peroxide when diluted because of the activation of the enzyme glucose oxidase, which oxidizes glucose to gluconic acid and hydrogen peroxide (Schepartz and Subers, 1964). Hydrogen peroxide is the major contributor to the antimicrobial activity of honey,and the different concentrations of this compound indifferent honeys result in their variable antimicrobial effects (Molan, 1992).Moreover, non peroxide factors also play critical role. The content of non peroxide factors are related to the floral source and sometimes account for the major part of the antibacterial activity in honey(Molan and Russell, 1988). Forty samples of different honey types (Acacia, Ziziphus , Brassica and Citrus) were collected from different areas of Pakistan and analyzed for moisture, pH, total acidity, ash, electrical conductivity, hydroxyl methyl furfural (HMF), sucrose, total sugars, invert sugar, protein, proline contents as well as macro and micro elements.The variation in composition of honey samples was observed due to different types of flora. Likewise, a significant level (P ; 0. 05) of ash, electric conductivity, sucrose, total sugar as well as macro and micro elements was also found in these honey types. Different formulations of honey has significantly inhibited growth of pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger when compared to control group, which is an evidence that honey is a therapeutic agent being used since ancient time throughout the world. Feng et. al. 1994) The discrepancy in the observed antibacterial activity can be due to several reasons. One possibility might be related to the differences in susceptibility of each species of microorganism to the antibacterial activity of honey used. Similar observations are reported by others (Nzeako and Hamdi 2000; Ceyhan and Ugur 2001; Taormina et al. 2001). As reported by others (Melissa et al. 2004) dilution of honey enhances hydrogen peroxide mediated antibacterial activity may explain some of the discrepancies of observed with the antibacterial activity of these honey.The presence of unstable putative agents and/or thermolabile antibacterial agent(s) could also be inactivated during the experimental procedure and thus may be considered as possible explanation of the observed insensitivity of some honey samples found in the preliminary study. 4. ) MATERIALS AND METHOD 4. 1) Materials Several local brands of honey and Dabur honey collected from different sources is used in this study. Also the honey is collected from various apiaries from- Pathankot(Punjab) and Saharanpur (Up). In the antibacterial study, several bacterial species known to be pathogenic to human such as E. oli. , S. aureus and Burkholderia capicium and Bacillus subtilis was used. These strains were obtained from biote chnology lab. in accordance to given requirement. Glass wares used:- Conical flask (500 milliliter), Conical flask (100 milliliter), Petri plates, glass rod, test tubes spreader, glass beakers, Glass slides and cover slip, Plastic wares used:- Beakers, Conical flask (500 milliliter), Conical flask (100 milliliter), dropper, appendrof tubes (2ml), auto pipette tips (10? l, 100? l, and 1000? l) Instruments used:- Hot air oven, Autoclave machine, Laminar air flow hood, Incubator, Microwave ovenOther material used:- Sprit lamp, cork borer,test tube stand, auto pipette (10? l, 100? l, and 1000? l), parafilm wax,,filter paper Chemicals used:- Nutrient agar, nutrient broth, Mueller Hinton agar, 70% Methanol, 100% methanol, ethyl alcohol ,sodium chloride. 4. 2) Methods Physicochemical study: Appearance of different honeys was observed. pH is determined using conventional procedure like using ph scale. Antibacterial study: Antibacterial study was carried out in steps. In the first step, an i n vitro screening will be carried out using either disc diffusion or well diffusion method.Well diffusion was carried out using plate diffusion, which I preferred. Preparation of test materials: Test materials will be prepared by diluting each honey at different dilutions, 25 ? l/100 ? l, 50 ? l/100 ? l, 75 ? l/100 ? l and one with no dilution. Moreover, net honey was also used as test material. All dilutions were carried out with double distilled and deionised sterilized water. 3. 2. 1) Source of bacterial strains The E. coli. , S. aureus, Burkholderia capicium and Bacillus subtilis were revived from the stock available in the various Biotechnology laboratories of Lovely Professional University, Phagwara (Punjab), India.Sub-culturing of bacterial strains in nutrient broth:- 100ml nutrient broth was prepared and ten test tubes were taken, autoclaved, and after this the Bacterial strains was inoculated in the nutrient broth in different test tubes and then incubated for 24 hours in i ncubator . Sub-culturing of the bacterial strains on solid media:- For All Bacterial strains:- 5. 6g of Nutrient agar was added to the 200ml of purified water. Then solution was heated and boiled for 1 minute to completely dissolve the powder. Then media was autoclaved.Then media(10-15 ml) was poured in Petri plates. Then Bacterial strains were added to media. Then Petri plate were kept in incubator for 18-48 hours at 37 degree C. Saline preparation : Take . 58g of Nacl and dissolve it to 10ml of distled water and make saline before 15 min of spreading and take the colonies from the nutrient agar plates and mix properly in the saline. Nutrient agar medium for antimicrobial testing: 7g of nutrient agar medium was added to the 250 ml of distilled water and heated for 1 minute to complete dissolve medium then autoclaved .After that pouring the petriplates and left it 20 minute for solidify medium and make wells , after solidify put the different Honeys in the wells and kept it to incub ate for 18-48 hours at 37C. Testing of antibacterial activity using agar well diffusion method: The bacterial strains were inoculated into 10 ml of sterile nutrient broth, and incubated at 37  °C for 18 h. Each culture was then spreaded on the surface of sterile nutrient agar plate and also pour-plated in nutrient agar media to perform the test in triplet, one with the control and the other two ith the test sample. Making the wells With the help of a cork borer make wells in the agar carefully without tearing the gel. In two agar plate of all four sets, four wells were prepared with the help of sterilized cork borer. Then with the hep of micropipette put 100 micro litre of honey sample into the wellIn the wells of two plates of each set, samples of following concentrations: (1) 100% sterile Honey(2) 75% sterile Honey(3) 50% sterile Honey and (4) 25% sterile Honey; were added by using micropipette. Also in the a well water was added as negative control.Then with the help of micropi pette put 100 micro litre of honey sample into the well Replace the lid of the plate between putting sample into wells to minimize exposure to air-borne contaminants. Cover the lid tightly with paraffin tape to avoid contamination. Incubation of the plates . A temperature range of 35 °C  ± 2 °C is required for 24-48 hours. Do not incubate plates in carbon dioxide  as this will decrease the pH of the agar and result in errors due to incorrect pH of the media. Measuring zone of inhibition 1.Following incubation, measure the zone sizes  to the nearest millimeter  using a ruler or caliper; include the diameter of the well in the measurement . 2. All measurements were made with the unaided eye while viewing the back of the petri dish. Hold the plate a few inches above a black, nonreflecting surface illuminated with reflected light . 3. Record the zone size on the recording sheet. Figure1: Testing of antibacterial activity using agar well diffusion method 4. )RESULTS Sub-cultu ring of bacterial strains in nutrient broth:- Figure2: Culture E. coli. , S. ureus and Burkholderia capicium and Bacillus subtilis in Nutrient broth media. Sub-culturing of the bacterial strains on solid media:- Fig. –3 E. coli subcultured on nutrient agar Fig. – 4 B. Subtilis subcultured on nutrient agar Testing of antibacterial activity using agar well diffusion method: Fig 5 : No antibacterial activity seen as no honey is poured (taken as negative control) in which E. coli is grown Fig 6 : No antibacterial activity seen as no honey is poured (taken as negative control) in which Bacillus subtilis is grown ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST E. coliFIG. 7 – ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY(pathankot sample) IN E. coli: 100% = 2. 6cm, 75%= 1. 9cm, 50%= 1. 7cm,and 25%= 1. 2cm FIG. 8– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY (dabur sample) IN E. coli : 100% = 2. 2cm, 75%= 1. 6cm, 50%= 1. 3cm, and 25%= 1. 1cm FI G. 9– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY (saharanpur sample) IN E. coli: 100% = 2. 8cm, 75%= 2. 4cm, 50%= 2. 2cm, and 25%= 1. 7cm; ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST B. subtilis FIG. 10– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY IN B. subtilis (pathankot sample): 100% = 3. cm, 75%= 2. 7cm, 50%= 2. 7cm, and 25%= 2. 5cm FIG. 11– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in B. subtilis (dabur sample): 100% = 2. 7cm, 75%= 2. 5cm, 50%= 2. 4cm, and 25%= 2. 2cm; FIG. 12– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in B. subtilis (saharanpur):100% = 3. 2cm, 75%= 2. 7cm, 50%= 2. 3cm, and 25%= 1. 5cm ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST Burkholderia capacium FIG. 13– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in Burkholderia capacium (pathankot sample): 100% = 2. 1cm, 75%= 1. 9cm, 50%= 1. 6cm, and 25%= 1. 4cm; FIG. 4– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY Burkholderia capacium (dabur): 100% = 2. 4cm, 75%= 1. 7cm, 50%= 1. 5cm, and 25%=1. 2cm FIG. 15– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY Burkholderia capacium (saharanpur):100% = 2. 5cm, 75%= 1. 9cm, 50%= 1. 7cm, and 25%=1. 4cm; ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST S. Aureus FIG. 16– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY S. aureus (pathankot):100%=2. 1cm, 75%= 1. 7cm, 50%= 1. 4cm, and 25%=1. 2cm FIG. 17– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY S. aureus (dabur):100% =3. 0cm, 75%= 2. 7cm, 50%= 2. 2cm, and 25%=1. cm FIG. 18– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY IN S. aureus (saharanpur): 100% = 2. 9cm, 75%= 2. 5cm, 50%= 2. 0cm, and 25%=1. 6cm Appearance Appearance of each of honey brand was examined and it was observed that honey were brown to dark brown in color, whereas other honey were golden yellow in color. Dabur honey- golden colour,transparent Pathankot sample – golden colour wit h sufficient transparency Saharanpur sample – dark yellow colour with zero transparency The transparency is due to processing of honey,while less transparent samples are unprocessed and taken directly from bee hive.Table 1- Diameter of the Zone of Inhibition by different concentration of Pathankot honey sample on different Bacterial strains. Bacterial strains| Zone of Inhibition (in cm)| | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. coli. | 0. 0| 2. 6| 1. 9| 1. 7| 1. 2| Bacillus subtilis| 0. 0| 3. 0| 2. 7| 2. 7| 2. 5| S. aureus| 0. 0| 2. 1| 1. 7| 1. 4| 1. 2| Burkholderia capicium| 0. 0| 2. 1| 1. 9| 1. 5| 1. 4| Table2- Diameter of the Zone of Inhibition by different concentration of dabur honey sample on different Bacterial strains.Bacterial strains| Zone of Inhibition (in cm) | | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. coli. | 0. 0| 2. 2| 1. 6| 1. 3| 1 . 1| Bacillus subtilis| 0. 0| 2. 7| 2. 5| 2. 4| 2. 2| S. aureus| 0. 0| 3. 0| 2. 7| 2. 2| 1. 8| Burkholderia capicium| 0. 0| 2. 4| 1. 7| 1. 5| 1. 2| Table 1- Diameter of the Zone of Inhibition by different concentration of Saharanpur honey sample on different Bacterial strains. Bacterial strains| Zone of Inhibition (in cm)| | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. oli. | 0. 0| 2. 8| 2. 4| 2. 3| 1. 7| Bacillus subtilis| 0. 0| 3. 2| 2. 7| 2. 3| 1. 5| S. aureus| 0. 0| 2. 9| 2. 5| 2. 0| 1. 6| Burkholderia capicium| 0. 0| 2. 5| 1. 9| 1. 7| 1. 4| In the preliminary screening process was observed that some honey brands have more while some other have less antibacterial activity. Some honey showed antibacterial effect against E. Coli and some showed strongest activity against Bacillus subtilis ,S. aureus and Burkholderia capicium. Clear zones of inhibition were produced by concentrated without dilution.Through the analysis of average values of the diameters of each honey, it is possible to infer a possible pattern in which commercial honeys, such as DABUR honey, have a greater average diameter while the homemade honeys from different regions, have a lower average diameter even at less concentrations, but the best antimicrobial activity was shown by Saharanpur honey sample with was collected directly from the apiary without any processing and it showed clear and large inhibition zones for all bacterial strains which were used for this test.Though after the dilutions were made, the low concentrated samples of Saharanpur honey resulted in rather poor antimicrobial activity. The wells in which water was loaded shoed absolutely no antimicrobial activity which was used as negative control. The results also showed that lower concentration of honey made through dilution shows less antimicrobial activity than concentrated ones. Thus honey are definitely effective against the bacterial strains at all concentration s. 6) REFERENCES Bibi S, Husain SZ, Malik RN (2008). Pollen analysis and heavy metals detection in honey samples from seven selected countries. Pak. J. Bot. 40(2): 507-516 * Cooper R. A. , Molan P. C. , Harding K. G. (1999) Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds, J. R. Soc. Med. 92, 283–285. * Cooper R. How does honey heal wounds? In: Munn P, Jones R,editors. Honey and Healing. UK: International Bee Research Association; 2001. * de Jong H. J. 1999) The Land of Corn and Honey:The keeping of stingless bees (meliponiculture) in the ethno-ecological environment of Yucatan (Mexico) and El Salvador, Utrecht UniversityPress, Netherlands, 423 p. * Kamal A, Raza A, Rashid N, Hammed TG, Lami M, Gureshin MA,Nasim K (2002). Comparative study of Honey collected from flora of Pakistan. On Line J. Biol. Sci. 23(9): 626-627. * Khalil MI, Shahjahan M, Absar N (2006). Glycemic Response and Lycemic Index of Bangladeshi Honey in Type 2 Diabe tic Patients. Malaysian. J. Pharm. Sci. 4(1): 13–19. * Lusby PE, Coombes AL, Wilkinson JM.Bactericidal activity of different honeys against pathogenic bacteria. Arch Med Res 2005; 36: 464-467 * Molan P. C. (1997) The antibacterial activity of honey. The nature of the antibacterial activity,Bee World 73, 5–28. * Mundo MA, Padilla-Zakour OI, Worobo RW. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. Int J Food Microbiol 2004; 97: 1-8. * Tumin N, Halim NA, Shahjahan M, Noor Izani NJ, Sattar MA,Khan AH, et al. Antibacterial activity of local Malaysian honey. Malaysian J Pharma Sci 2005; 3: 1-10.

Civil Rights Movements

Who Is It? Throughout the Civil Rights Movement, Martin Luther King Jr. played a crucial role in organizing many nonviolent events such as the March on Washington and Selma to Montgomery March. These events eventually influenced the Congress to pass both the Civil Rights Act of 1964 and the Voting Rights Act of 1965. The assassination of Martin Luther King Jr. also led to dramatic impacts on later laws. Martin Luther King Jr. s the main reason why the 1960s US Civil Rights Movement succeeded, as he fought against de jure segregation and led to the changing of laws; however, this is justified only to a great extent, for he contributed little to social activism. Martin Luther King Jr. successfully influenced many government reforms through the act of nonviolence during the Civil Rights Movement.He was elected president of the Southern Christian Leadership Conference in 1957, a nonprofit organization aiming to redeem â€Å"the soul of America† through nonviolent resistance. In 19 63, Martin Luther King Jr. ed a nonviolent campaign aimed at Birmingham, Alabama, which was known as the one of the most segregated city in the United States during that time. During this campaign, Martin Luther King Jr. advocated nonviolence in â€Å"Letter From a Birmingham Jail†. Later on, the SCLC decided to use young children in demonstrations. However, more than 1,000 children were attacked by police troops. The images of children being assaulted by dogs and water hoses were captured by photographers, which led to a â€Å"national outrage resulting in a push for unprecedented civil rights legislation†.This event drew attention to segregations in the South, and promoted sympathy for civil rights protestors across the nation. Over the next few years, Martin Luther King Jr. organized the March on Washington for Jobs and Freedom in and the Selma to Montgomery March. During the March on Washington, Martin Luther King Jr. delivered the famous â€Å"I Have A Dream† speech. This march was a success-   the speech cemented Martin Luther King's â€Å"status as a social change leader†, helped inspire people to act on civil rights, and publicized the US Civil Rights Movement across the world.This event also led to the passing of the Civil Rights Act of 1964, an act that banned all forms of discrimination. The next year, Martin Luther King Jr. organized the Selma to Montgomery March to fight against African American disenfranchisement. Following the march, the Congress passed the Voting Rights Act of 1965 that got rid of the remaining obstacles to voting for African-Americans. By organizing nonviolent marches and campaigns, Martin Luther King Jr. was successful in motivating the passing of important laws that improved problems caused by segregation.The assassination of Martin Luther King Jr. was one of the major reasons that led to the Civil Rights Act of 1968. Before the act was passed, white people used segregate against minority groups by forming restrictive covenants and practicing gerrymandering. After Martin Luther King Jr. was assassinated on April 4th, 1968, riots erupted over the nation. President Lyndon B. Johnson called on the Congress to pass the Civil Rights Act of 1968, hoping to stop the riots and pacify angry protesters. This act banned all forms of discrimination in housing sales and rentals. Martin Luther King Jr. as able to influence several acts passed by Congress even after he died, which proves that he is a major reason why the US Civil Rights Movement was successful. Despite Martin Luther King's efforts to fight against de jure segregation, he contributed little to ending de facto segregation. Malcolm X, an African American who also played a key role during the Civil Rights Movement, had different a different way to fight against segregation. He promoted active resistance and violence. As James Cone, the author of Martin & Malcolm & America says, â€Å"Before Malcolm came along, we were all N egroes. After Malcolm, he helped us become black†.Malcolm X taught African Americans to be proud of their heritage, and changed how black people thought about themselves. Several of his followers gathered together and formed the Black Panther Party, and organization that had the same objectives as Martin Luther King Jr, but were willing to use violence to solve the problem. By promoting black power and increasing black voter registrations and Afrocentrism, Malcolm X was able to build confidence in blacks and encourage them to take pride in their African heritage, which shows that he contributed to the success of the US Civil Rights Movement as well.Overall, Martin Luther King Jr. was a major reason for the success of the US Civil Rights Movement. He organized campaigns and marches that inspired people to take action, and influenced the Congress to pass important acts. His death led to the Civil Rights Act of 1968 as well. However, he wasn't the only one who made all of this po ssible. Malcolm X and encourage blacks to be proud of their African heritage, and increased black voter registrations. Thus, Martin Luther King Jr. is the main reason why the 1960s US Civil Rights Movement succeeded, yet this is justified only to a great extent.